Coding

Part:BBa_K3793006:Design

Designed by: Rupesh Balaji Jayachandran, Enrique de Dios Mateos   Group: iGEM21_Aalto-Helsinki   (2021-10-21)


CDS - Human AhR Interacting Protein


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 439
    Illegal PstI site found at 652
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 439
    Illegal PstI site found at 652
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 53
    Illegal BamHI site found at 686
    Illegal BamHI site found at 953
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 439
    Illegal PstI site found at 652
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 439
    Illegal PstI site found at 652
    Illegal AgeI site found at 578
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 779


Design Notes

The residues comprising of the different domains were to be identified for creating a truncated version. The restriction sites of type IIS enzymes like BsaI and BbsI were to removed from the CDS. An N-Terminal 6x HIS tag has been added to facilitate downstream purification.


Source

The sequence is modified from the NCBI entry of AIP (NM_003977.4)

References